vacutainer blood collection procedure

In most cases, another puncture in a site below the first site is advised. Transfer Device 6. ESG (Environmental, Social, and Governance), Policies, Guidelines and Statements Center, Our live chat is available between the hours of 8.30am - 5.00pm EST, Monday - Friday, BD Original Equipment Manufacturing (OEM), Patient Care Support Across the Continuum. 0000015023 00000 n Now ensure the patient what is to be done. endobj 0000181762 00000 n tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Withdraw the needle slowly, and tell the patient to place a cotton swab over the wound until the blood ceases. /Type/ExtGState At no time may phlebotomists perform venipuncture on an artery. Tubes often include additives that mix with the blood when collected, and the color of each tube's plastic cap indicates which additives it contains. 0000010912 00000 n Place a gauze pad over the puncture site and remove the needle. BD 362725 EST Plastic Tube 3ml with Pearlescent White Hemogard Closure 10 [Pack of 1000] Excl. A lab coat or gown must be worn during blood collection procedures. 0000036227 00000 n 0000014014 00000 n BD's trade name for the blood handling tubes is Vacutainer. Used for Genetics testing. 5.4 Collect blood into three (3) 8ml CPT tubes per sample using the standard technique for BD Vacutainer Evacuated Blood Collection Tubes (see Venipuncture Technique & Sample Collection section and Prevention of Backflow section). The tube must be labeled before leaving the patient. The plastic tube version, known as Vacutainer PLUS, was developed at B-D in the early 1990s by E. Vogler, D. Montgomery and G. Harper amongst others of the Surface Science Group as US patents 5344611, 5326535, 5320812, 5257633 and 5246666.[15]. Carefully Remove the needle from the syringe and gently expelthe blood into the appropriate vial or container. All other items used for the procedure must be disposed of according to proper biohazardous waste disposal policy. Now decide the amount of blood required and select the appropriate vials or containers according to the tests to be done. endobj Position the patient with the arm extended to form a straight-line form shoulder to wrist. This latex-free, single-use device Compare this item Volume - 3ml. 6. A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. BD Vacutainer plastic tubes offer a safe method of blood collection and reduce the potential for tube breakage and specimen spillage, thereby reducing the potential for exposure to bloodborne pathogens. Allow each tube to fill up with the adequate amount of blood required for the lab tests ordered. and syringe, avoid drawing the plunger back too forcefully. If commercially available tubes are to be used, the researcher should use the red topped tubes. /Subtype/Image 2. The label must include the patient first and last names, DOB,, collection date and time and collectors initials. Tubes with different additives are used for collecting blood specimens for specific types of tests. MATERIALS A. The BD Vacutainer blood collection needles & sets for collecting venous blood. stream Prior to collection, consult the specific test ordered to determine the proper tube type and specimen volume required. 0000003106 00000 n A patient should never be stuck more than twice unsuccessfully by a phlebotomist. into the tubes; do not use the plunger on the syringe to force . 2. 0000015567 00000 n Guide to using the BD Vacutainer Blood Collection Set NHS Fife 1.93K subscribers Subscribe 18K views 3 years ago Health & Safety Manager, Craig Webster, gives advice on the safe use of the BD. A Blood test is prescribed in most of the blood-related disorders, metabolic disorders and in various infections as well as for the Regular health Check-ups. 0000010428 00000 n 3. Save my name, email, and website in this browser for the next time I comment. With the left hand, hold the patients arm so that the skin over the vein gets tightened and more prominent. Holding the needle in line with the vein, use a quick, small thrust to penetrate the skin and vein in one motion. Gloves are to be worn during all phlebotomies, and changed between patient collections. *NOTE: Discard blood can be used in blood culture collection sample if CVL is not antibiotic "locked". 155 48 Blood collection tubes expire because over time the vacuum is lost and blood will not be drawn into the tube when the needle punctures the cap. Your email address will not be published. This blood will be used for staining. 0000013853 00000 n If anticoagulant is used, gently shake the vials for proper mixing of the blood with the anticoagulant. /FunctionType 0 0 )jVdZV46 }J]UV\e|}*>O\,qTC\q\/'rYR.>v 6EoO^jUqU],?B`|9N]7K!A*K i6]IRwLvHH&UnbQ4 ,oluQzO#L_n(^}Qa$}T-o?%RrcSI+VBIQhUD*n5g1k.Tkmm/u|gAz-+)m[dmNjKU Disposable, single use needle holder C. Evacuated blood collection tubes D. Tourniquet (non-latex), disposable single use Immediately apply slight pressure to the gauxe pad over the venipuncture site.. 12. 9. 21 G x .75 in. BD Vacutainer Safety-Lok Blood Collection Set with 7 in. Usually drawn first for minimal risk of contamination. The Veterinary Surgeon shall enter the swab card number on each of the vacutainer tubes; 3. B. An OSHA acceptable, puncture proof containermarked Biohazardous. 0000182132 00000 n 0000099961 00000 n 0000014709 00000 n 2023 BD. /Height 64 Procedure 1. Extensive scarring or healed burn areas should be avoided. How to buy Reach out to our team for ordering information. <>stream 5. Blood Collection Tubes. Practical Nurse Program October 10, 2019 1 NFDN 2003 Phlebotomy Collection Procedure Check Doctors orders for allergies Assemble the necessary equipment Needle (vacutainer adaptor needle) Blood Collection Tube(s) - correct color and number of tubes based on tests ordered Tourniquet Alcohol Prep Pad Gauze Tape Clean gloves Labels Sharps Requisition and tube labels Thoroughly wash hands . The color of the top indicates the additives in the vial. The filled tube is then removed and another can be inserted and filled the same way. 0000014235 00000 n 4. XUwpYfU!%JeU{ B $@ a$44q hhzgvv7v^{kLfEsK=*D~so1(c5Z0k0P$6!_ Chg$XFUl5 << Red Top (serum): Plain and gel. Main Line Health Laboratories provides the following standard procedure for venous blood collection. <> Dispose of the syringe and needle as a unit into an appropriate sharps container. 2023 BD. With the right hand, Grasp the vacutainer unit (Vacutainer holder+needle) and remove the translucent plastic cover from the needle. 0000001256 00000 n 0000011086 00000 n Ensure that the collection tube is completely pushed onto the back of the needle in the hub. If the unit and needle are not held steady while pushing in the tube, the needle may slip out of the vein or puncture the opposing vein wall. These tubes are used primarily for chemistry tests. BD Vacutainer CPT is a fully-closed system for separation of mononuclear cells from whole blood, where cell separation is carried out in the primary blood collection tube. /Size[2] 4. Now, remove the Tourniquet, ask the patient to open the fist and place the cotton swab over the needle and wound. It is also offered with a preattached holder to add convenience and help ensure OSHA single-use holder compliance. The Supervisor should be called to assess the patient. E. Holding the hub securely, insert the first vacutainer tube following proper order of draw into the large end of the hub penetrating the stopper. 6. Study Guide and Procedure Checklist Manual Package: An Applied Learning Approach, 13th Edition . Lavender or Pink Top (plasma): Contains EDTA. /Range[0 1 0 1 0 1 0 1] Contaminated surfaces must be cleaned with freshly prepared 10% bleach solution. Tubes containing gel can be easily handled and transported after centrifugation without the blood cells and serum mixing. 5. A. Reassure the patient that the minimum amount of blood required for testing will be drawn. stream Individually packaged 70% isopropyl alcohol wipes. 0000010686 00000 n /TR/Identity <> Used primarily for hematology and blood bank testing. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Blood collection procedures should be approved by the institutional ethics committee (AWERB, IACUC or equivalent) and detailed in the approved study protocol. gr @5c3Ok=3. 13x75 mm 2.4 mL BD Vacutainer glass plasma ESR tube. startxref A lab coat or gown must be worn during blood collection procedures. The safety mechanism can be activated immediately after the blood draw and helps protect against needlestick injury. Used for chemistry and reference tests. "kB#i]#}= -XIYFIO^qpr;aR Ask the patient to apply pressure for at least 2 minutes. 0000013645 00000 n The BD Vacutainer CPT Cell Preparation Tube with Sodium Citrate is an evacuated blood collection tube system containing 0.1 M sodium citrate anticoagulant and blood separation media composed of a thixotropic polyester gel and a FICOLL Hypaque solution. The BD Vacutainer blood transfer device is designed with healthcare workers' safety in mind. The safety mechanism can be activated immediately after the blood draw and helps protect against needlestick injury. << 4 0 obj 23 G x .75 in. 14. Do not attempt a venipuncture more than twice. Single use, disposable, latex-free tourniquets. All surfaces are cleaned daily with bleach. Place the tips of the index and middle fingers of the right hand behind the flange of the vacutainer holder. If using a needle. Vacutainer tubes may contain additional substances that preserve blood for processing in a medical laboratory. If an IV is in place, samples may be obtained below but NEVER above the IV site. 9. How To Manage Clinical And Related Waste. Vacutainer technology was developed in 1947 by Joseph Kleiner,[2] and is currently marketed by Becton Dickinson (B-D). Needles are never recapped, removed, broken, or bent after phlebotomy procedure. Attach the appropriate needle to the hub by removing the plastic cap over the small end of the needle and inserting into the hub, twisting it tight. POLICY: By establishing a procedure for the correct collection of blood by venipuncture many pre-analytical errors and patient management complications can be avoided. UF Health Pathology Laboratories Nontuberculosis Mycobacteria Laboratory, This page uses Google Analytics (Google Privacy Policy). All rights reserved. Avoid probing for the vein. For Research Use Only. V(5/^O[$thW)Y".`JET(5:rSG H;_=6V3b-C+364,--N`T S`"Mv"T4JU )IMK_V ?RS\SV & >b~5dU 3v:*[;"-*Kb>I1 OI(lQZa@UJI%G\XvTNcJl]XR~zb-Xu h Jhic4 Lavender BD Hemogard closure. 0000011440 00000 n A sales representive will get in touch with you shortly. How to Give Yourself a Spa-Like Facial Massage and Lymphatic Drainage? Refer to Pediatric blood culture procedure. Vacutainers are widely used in phlebotomy in developed countries due to safety and ease of use. D. Holding the needle in line with the vein, use a quick, small thrust to penetrate the skin and enter the vein in one smooth motion. 3. >> /Filter[/FlateDecode] /Filter[/FlateDecode] Verify that any diet or time restrictions have been met. xref 0000181610 00000 n BD supports the healthcare industry with market-leading products and services that aim to improve care while lowering costs. Lay the arm of the patient on the table. Phlebotomy is a technique in which a needle is temporarily inserted into a vein to provide venous access for venous blood sampling. Firstly ensure that the patient should be in the comfortable position. G. When blood flow stops, remove the tube by holding the hub securely and pulling the tube off the needle. 6. 0000009374 00000 n 2. We host and take part in events that excel in advancing the world of health. (5 ways), Signs That Your Period is Coming Tomorrow: 6 Vital Signals, Care and Maintenance of Laboratory Equipment. After collection of the whole blood, allow the blood to clot by leaving it undisturbed at room temperature. The vacuum tubes are designed to draw a predetermined volume of blood. 6%L)v`"V$gdHI"@lBm Gray Top (plasma): Contains sodium fluoride/potassium oxalate. 9. 0000001840 00000 n %PDF-1.5 For the Separation of Mononuclear Cells from Whole Blood Sterile Interior 8 mL Draw Capacity (16 x 125 mm Tube Size) VDP40393(02) 362753 2020-05 INTENDED USE The BD Vacutainer CPT Cell Preparation Tube with Sodium HeparinN is an evacuated Tube intended for the collection of whole blood and the separation of mononuclear cells. This procedure is one example of a variety of methods that can be used to activate platelets. BD and the BD Logo are trademarks of Becton, Dickinson and Company. (According to OSHA, "removing contaminated needles and All other tubes containing an additive should be gently inverted 8-10 times. a BD Vacutainer Safety-Lok Blood Collection Set. 3. Venipuncture can be done by two methods:
2. 7. The Vacutainer needle is double-ended: the inner end is encased in a thin rubber coating that prevents blood from leaking out if the Vacutainer tubes are changed during a multi-draw, and the outer end which is inserted into the vein. Observe universal (standard) safety precautions. endobj %PDF-1.5 % NOTE: When using a winged blood collection set for venipuncture and a coagulation tube is the first tube needed, first draw a discard tube (plain red top or light blue top). tubing and no luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Collection of Diagnostic Venous Blood Specimens - Clinical and Laboratory Standards Institute 2017 Henry's Clinical Diagnosis and Management by Laboratory Methods E-Book - Richard A. McPherson . If no patient labels are available, manually label the tubes with the required information. Assemble the necessary equipment appropriate to the patients physical characteristics. BD promotes clinical excellence by providing various resources on best practices, clinical innovations and industry trends in healthcare. Blood Collection Process: Venipuncture Venipuncture Procedure PDF ROUTINE VENIPUNCTURE GUIDELINES MATERIALS 1. This usually takes 15-30 minutes. Used by chemistry for glucose testing. (adsbygoogle = window.adsbygoogle || []).push({});
, Needle gauge For Adults: 20-22 G & For Children: 23-24 G, Check out the blood collection by Capillary puncture. Release tourniquet and collect blood into a labeled ACD VACUTAINER tube. The larger median cubital, basilic and cephalic veins are most frequently used, but other may be necessary and will become more prominent if the patient closes his fist tightly. The single-use device reduces the risk of transfer-related injuries, while maintaining the specimen integrity required for accurate results. This decreases the complexity of steps for mononuclear cell separation, thereby minimizing variability from sample processing. endobj Avoid drawing blood from a hematoma. 2. Notify your supervisor or patients physician if unsuccessful. Apply the tourniquet 3-4 inches above the collection site. All rights reserved. 1 We host and take part in events that excel in advancing the world of health. 0000218054 00000 n Up]Inm$.q[ Q>>Ocm1$'C3+BH&zn7bgRpq$MO^! We host and take part in events that excel in advancing the world of health. Most commonly used to draw the blood samples directly from the vein. endobj Perform activation and staining or fixation of platelets within 10 minutes of blood collection. Other products you might be interested in, BD Vacutainer Eclipse blood collection needle, BD Vacutainer push button blood collection set, BD Vacutainer UltraTouch push button blood collection set, Surgical Instruments and Sterilization Container products. Collect blood into the tube using the standard technique for BD Vacutainer Evacuated Blood Collection Tubes (see Venipuncture Technique & Sample Collection section and Prevention of BD Vacutainer Safety-Lok Blood Collection Set with 12 in. The BD EST tubes are plastic tubes and have no additive in them. 10 Best Needle Burner / Syringe Destroyers in 2023, How to Instantly Fall Asleep After Drinking Coffee? Now Assemble the vacutainer holder & needle by inserting short end of the vacutainer needle which is covered with the rubber coating into the threaded end of the holder. <>/OutputIntents[<>] /Metadata 580 0 R>> A range of Vacutainer tubes containing blood, Learn how and when to remove this template message, "Vacutainer and Their Use in Blood Sampling", "A golden age of clinical chemistry: 19481960", "Blood collection: routine venipuncture and specimen handling", "Effects of standard anticoagulants and storage procedures on plasma glucose values in seals", "BD Vacutainer Venous Blood Collection Tube Guide", "An Appraisal of the "Vacutainer" System for Blood Collection", https://en.wikipedia.org/w/index.php?title=Vacutainer&oldid=1136329583. [5][6][7], The term order of draw refers to the sequence in which tubes should be filled. Tubes with different additives are used for collecting blood specimens for . 21 G x .75 in. Sharps Disposal Container. tubing and luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. Identify the patient, two forms of active identification are required. A Vacutainer blood collection tube is a sterile glass or plastic tube with a closure that is evacuated to create a vacuum inside the tube facilitating the draw of a predetermined volume of liquid. >> tubing and no luer adapter features a forward-shielding safety mechanism that helps protect against needlestick injury. BD promotes clinical excellence by providing various resources on best practices, clinical innovations and industry trends in healthcare. BD offers training resources to help improve your clinical practices as part of our goal of advancing the world of health. This aid in the prevention of bruising and edema formation. 5. BD supports the healthcare industry with market-leading products and services that aim to improve care while lowering costs. 0000009632 00000 n If hands are not visibly contaminated a commercial foaming hand wash product may be used before and after each patient collection. `Vw)=l]( AxF ;|;J`zqi5/dj 4 0 obj endobj 4 0 obj This procedure establishes criteria for the proper collection of blood specimens. i$o.-N %0 $e5eC6X} &f V)Z|{as@l G9@(+3`'I'N =I*+L'n. [1, 2, 3] Veins have a three-layered wall composed of an internal endothelium surrounded by a thin layer of muscle fibers, which in turn is surrounded by a layer of connective tissue.Identification of the optimal site for venous access (see Technical . 2 0 obj 8. 21g or less 3. 1 0 obj use of an incorrect anticoagulant. 2 0 obj Place the needle bevel up in the line with the vein, decrease the angle of the needle until it is almost parallel to the skin surface direct the needle towards the vein and pierce the skin and vein wall at approximately at15-30 angle. The additives may include anticoagulants (EDTA, sodium citrate, heparin) or a gel with density between those of blood cells and blood plasma. Trace elements, heavy metals, most drug levels, This page was last edited on 29 January 2023, at 20:59. transfer device to fill vacutainer tubes, allow the vacuum to pull the blood. /ColorSpace/DeviceCMYK 7.1.4.5 Refer to BD Vacutainer Venous Blood Collection Tube Guide for complete listing and description: /Filter[/FlateDecode] Vacutainer tubes may contain additives designed to stabilize and preserve the specimen prior to analytical testing. Hi, Im the Founder and Developer of Paramedics World, a blog truly devoted to Paramedics. 10. tubing and pre-attached holder in a fully sterilized package, offering an integrated device that may simplify the phlebotomy procedure. hb```f``a`c`9 B@16%;00h9|9eqGW,"v5Ir'VJYTJ-YbggElZ9@I:Gx-Mj] 1 AEGGG BD Vacutainer EST tubes may be used as a discard tube, to remove the dead space from the tubing of blood collection sets. Continue exchanging the tubes until the required no. Mk venipuncture site is dry. A sales representive will get in touch with you shortly. This information must match the requisition. Implant Dentistry: A permanent solution to tooth loss, Top Tips to Build Self-confidence in Your Dyslexic Child, How To Use Hair Shampoo: Step-By-Step Guide for Best Results, The Importance Of Concussion Education For Medical Professionals, 10 Facts About Artificial Tooth Replacement That Will Make You Smile. Needles and hubs are single use and are disposed of in . Screw it tightly using a clockwise motion. Related Products: Edta Tube. /Range[0 1 0 1 0 1 0 1] dA 1. Blood should flow into the evacuated tube. <>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 25 G x .75 in. Only BD provides a full offering of blood collection tubes in both glass and plastic. Antiseptic. <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> 0000000016 00000 n With your left hand, slightlypull back the piston till the required amount of blood is obtained into the syringe. Ask the patient to state their name and date of birth. 5. Tick off the collected samples on the Blood Worksheet (Attachment 1) [1], Vacutainer tubes were invented by Joseph Kleiner in 1949. The tourniquet will be applied 3-4 inches above the selected puncture site and will not . Observe all applicable isolation procedures. 0000018520 00000 n 155 0 obj These tubes are used for coagulation tests and need to be completely filled to ensure the proper ratio of blood to anticoagulant. Activate the safety device and properly dispose of the vacutainer holder with needle attached into a sharps container. Place a gauze pad over the puncture site and quickly remove the needle. 1. The meanings of the various colors are standardized across manufacturers. /Domain[0 1] BD offers training resources to help improve your clinical practices as part of our goal of advancing the world of health. BD and the BD Logo are trademarks of Becton, Dickinson and Company. <> BD Vacutainer PPT Plasma Preparation Tubes. 0000003048 00000 n Required fields are marked *. 0000009793 00000 n (adsbygoogle = window.adsbygoogle || []).push({}); Paramedics World was founded in 2017 by Sahil Batra, with the sake to provide medical and paramedical information and reference meterial to the students and the professionals. /FunctionType 0 %PDF-1.5 4. /Length 22 4. If multiple tubes are needed, follow the proper order of draw. 0000034815 00000 n Push the tube all the way into the holder with your thumb until the needle completely punctures the rubber stopper of the vacutainer tube. 0000011799 00000 n Vacutainer tube holder 5. #labtechniciancourse #laboratory #bloodcollection #labtech #bloodsample #bloodbank #collection #trending #viral #viralvideo #video #metropolis #hospital 0000001963 00000 n Your email address will not be published. BD Vacutainer Safety-Lok Blood Collection Set with 12 in. Vacutainer Blood Transfer Device BD Using a syringe needle to transfer venous blood to a blood collection tube or blood culture bottle is both a dangerous procedure and an OSHA-prohibited practice. 2 0 obj A vacutainer blood collection tube is a sterile glass or plastic test tube with a colored rubber stopper creating a vacuum seal inside of the tube, facilitating the drawing of a predetermined volume of liquid. v1Znrch6j]6f~NcD!~-+ @ejLcPe&[c We support our products with extensive clinical expertise and educational materials to help you achieve the best possible results. x][s~wgRE\-gNi0${je"6 A`Y'3 FC>Ww>nW\]:}]m/(?}rWV0^_? When bleeding stops, apply a fresh bandage, gauze or tape. These additives are typically thin film coatings applied using an ultrasonic nozzle. 7. ]qEtftP $0{~5~d4]IFw#!5\jCCv'An.F]Gw $h/E$9BonCE%.RY@O"@/a* qm'I endobj 0000015000 00000 n >> DO NOT SHAKE OR MIX VIGOROUSLY. 11. [16] In developing countries, it is still common to draw blood using a syringe or syringes. 1. Place arm in a downward position 8. Select the appropriate vein for venipuncture. BD VacutainerSafety-Lok blood collection set. Begin with a quality specimen for quality results. BD Vacutainer Safety-Lok Blood Collection Set with 12 in. Now, Disinfect the selected site for puncture using the Alcohol swab. SST PST EDT A S . Green Top (plasma): With and without gel, contains lithium heparin. /Type/XObject Gently invert tubes containing an additive 5-8 times. 0000010186 00000 n The Vacutainer Blood Collection Tubes, both plastic and glass, are engineered to reduce specimen spillage.The Safety-Lok Blood Collection Sets and the Eclipse Blood Collection Needles feature a patented . << 23 G x .75 in. ZAJezAqPfI1-4o0]wh1_ea.wWnn;,7\[j@.4cj'DU)ai0A9v}p sh5TK@h %XZE#eCKnL- What to expect from early intervention orthodontics? <> BD's collection of literature on industry and on our offerings gives you information you can use to continue striving for excellence. 11. 0000011747 00000 n Features and Benefits Glass and plastic options 23 G x .75 in. Ask the patient to make the fist. BD Vacutainer Safety-Lok Blood Collection Set with 12 in. If more than one specimen is needed, push another tube into the holder. 0000005296 00000 n The BD Vacutainer CPT Tube with Sodium HeparinN should be at room temperature (18-25 C) and properly labeled for patient identification. 2. ESG (Environmental, Social, and Governance), Policies, Guidelines and Statements Center, Our live chat is available between the hours of 8.30am - 5.00pm EST, Monday - Friday, BD Original Equipment Manufacturing (OEM), Patient Care Support Across the Continuum. 5. endobj If using a blood. 15. [14] The Vacutainer was preceded by other vacuum-based phlebotomy technology such as the Keidel vacuum.

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