dextran hydrodynamic A long-chain polymer molecule in solution takes on a somewhat kinked or curled shape, intermediate between a tightly curled mass (coil) and a rigid linear configuration. ; Rjumtsev, E.I. Biophysical Journal, 27, 177-181. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Singh TRR, Garland MJ, Cassidy CM, Migalska K, Demir YK, Ryan SAE, Woolfson D, Donnelly RF. Pure Appl. Federal government websites often end in .gov or .mil. Ioan, C.E. European Polymer Journal, 37, 619-622. hydrodynamic Dextran Carbohydrate Polymers, 39, 377-380. Data File Dextran. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Theory, Estimation, Experiment, and Data. Fixable: Fixable via Lysine. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. WebHydrodynamic radius = variable size of an analyte with specific MW based on dynamical properties. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Journal of Applied Polymer Science, 72, 871-876. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should Materiaux 1-5. Biotechnology Progress, 19, 544-548. 2011;16(2):518. 2004;25(9):155967. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. Curley SA. Schematic illustration of transdermal delivery after the application of the microporation device. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. hydrodynamic The values of Mark-Houwink parameters could be universalized with certain precautions, being an indication for the calculation of molecular weight in the temperature range of 20-40C. (1999). Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. 4K and M.W. Charge: Anionic. Crini, G. (2005). Format), Citation-(BibTeX WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. principles of dynamic light scattering WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. Harding, Stephen E. (1997). The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. Progress in Polymer Science, 30, 38-70. Catiker, E.; Guner, A. For a limited temperature range, the viscosity of a polymer solution varies generally following a relation similar to that of usual liquids (rrhenius plot), where the polymer concentration is not very high and that temperature remains far enough from glass transition [38], and for system PEAorganic solvents see reference [63]. California Privacy Statement, Proc of SPIE. Advances in Cosmetic Surgery. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Biomaterials Research The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Carbohydrate Polymers, 79, 380-390. Environ Toxicol Pharmacol. European Journal of Pharmaceutics and Biopharmaceutics, 68, 319-329. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Cite this article. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Carbohydrate Polymers 72, 356-360. Kim J, Jang JH, Lee JH, Choi JK, Park WR, Bae IH, Bae J, Park JW. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. Article Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Progress in Biophysical Molecules Biological, 68, 207-262. With FITC-dextran 20 K, cumulative It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). Pore closure examination was performed using an optical microscope (BX43, Olympus, Japan) immediately (time 0), 4, 8, and 24h following device application. Protein Hydrodynamics with Light Scattering Nanoparticles in magnetic resonance imaging: from simple to dual A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. Durand, A. Comparison of single-point and dilution procedures. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). DLS tests were conducted at detection angle of 90 (standard) and 173 (backscatter) to measure protein hydrodynamic radius, while protein charge was examined in phase analysis light scattering (PALS) mode at detection angle of 15 (forward). WebTry out PMC Labs and tell us what you think. Gekko, K. (1971). Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Interaction Parameter (); Expansion Factor (); Steric Hindrance Factor (); and Shielding Function F(); for the System PEAOrganic Solvents by Intrinsic Viscosity Measurements. All possible degrees of curling can be displayed by any molecule, but there will be an average configuration which will depend on the solvent. Radiofrequency ablation of malignant liver tumors. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. Human skins (Full-thickness human skin) were supplied by HansBiomed. Banga AK. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Kasaai, Mohammad R. (2007). The micropore formed at the SC through to the epidermis, enabling drug delivery. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Macromolecules, 29, 2265-2268. Dilute Solution. Weissella: An Emerging Bacterium with Promising Health Benefits. II. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Int J Food Sci Nutr. For 40 kDa molecules, the hydrodynamic diameter d h ~8 nm is much smaller than the collagen mesh size [33,41]. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. dextran Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Regulating dynamic signaling between hematopoietic stem cells Garcia de la Torre, J.; Carrasco, B. 20K groups (n=3, *: p<0.05, **: p<0.01). The molecular weight and Simha number do not change in this temperature range (Mw from 8.8-200kDa), P slightly change, showing modifications in the hydrodynamic properties of the biopolymer in aqueous solution as [] and RH. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Huggins, Maurice L. (1942). Figure 2 shows the classical method for calculating Mark-Houwink parameters, where a linear relation between the intrinsic viscosity and the molecular weight is established for each temperature. In contrast to linear chains, power law behavior is observed only asymptotically at large Mv. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). ; Veldhuis, T.F.J. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. (2003). This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Format), Citation-(EndNote European Polymer Journal, 36, 2143-2146. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Drug deliv. Structure Properties of Dextran. Journal of Polymer Science, 17, 527-546. The measurements of dextrans molecular weight were determined by size exclusion HPLC (Gilson, France) with Refraction Index detector using a Polysep-GFC-P5000 column (7.8030 mm). (2003). (2004). 2012;9(2):14754. TEWL measurements were taken to assess the integrity of the skin barrier and the extent of SC disruption. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. The skin poration was performed using the microporation device (LG electronics, Korea). A few percent of R (13) glucosidic linkages provides side chains which appear to be short. Saudi Pharm J. FOIA Drug Deliv. Solvent effects on polysaccharide conformation. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Lee WR, Shen SC, Sun CK, Aljuffali IA, Suen SY, Lin YK, Wang JJ, Fang JY. Transdermal drug delivery: overcoming the skins barrier function. Carbohydrate Polymers, 38, 267-271. WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. For simplicity, macromolecules are treated as rigid molecules for a hydrodynamic study. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Polymers | Free Full-Text | Confinement-Induced Fractionation and The mechanism of the dextran-induced red blood cell Aggregation. Article (2003). FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Epub 2017 Jun 1. Food Funct. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. ; van Nostrum, C.F. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. All authors read and approved the final manuscript. Die Makromolekulare Chemie, 148(1), 229-238. 2015;32(5):170413. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Harding, Stephen E. (2005). Advances in Physical Chemistry, Vol. Critical parameters for gel permeation Expert Opin Drug Deliv. Guner, A.; Kibarer, G. (2001). Cookies policy. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. This result could reflect the presence of some aggregated species within the lysozyme sample solution. These structural differences can significantly affect FITC-dextran delivery due to even after microporation [30]. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich GYA primarily conducted research. Global hydrodynamic analysis of the molecular flexibility of galactomannans. 20K. * shows a significant difference between the M.W. Synthesis and characterization of blue dextrans. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows The Mark-Houwink a constant is close to 0.5 in poor solvents. Figure4 shows the histological analysis of micropores in human cadaver skin. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. The skin was treated with the RF-based microporation device, inducing micropore ablation through alternating electrical current. Bahary, W.S. In the range of working temperatures and molecular weights of dextrans, similar intrinsic viscosity results obtained by other researchers and published here are slight, as shown in tables 1 and 3. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. The .gov means its official. 2016;9706:97061E6. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Liu Zonghua; Yanpeng Jiao; Yifei Wang; Changren Zhou; Ziyong Zhang (2008). Influence of molecular weight on Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Amersham Biosciences, AA Edition, www.amershambiosciences.com. 2006;3(3):37385. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 The Mark-Houwink. Learn More. A simple way of universalizing the measurements is shown in figure 4 where there is a curve at each temperature for every molecular weight. Dilute solution properties and degree of chain branching The above data are not useful because it can not be compared with those obtained in this work. Colloid and Polymer Science, 286, 1223-1231. Hydrodynamic American Journal of Food Science and Technology 2013, 1(3), 60-66. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. These modifications include degradation of hemidesmosomes attached to the dermis, degradation of desmosomes linked to adjacent cells, contraction of intracellular tonofilament, and lamellipodia formation.
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